RootPath is a synthetic biology company that specializes in high-throughput gene synthesis and functional interrogation of genes. Their innovative PathFinder DNA Assembly™ technology allows for the assembly of long, functional DNA molecules from short DNA strands through a molecular programming approach. This method enables the simultaneous self-assembly of tens of thousands of short DNA strands into thousands of long genes with high accuracy, significantly reducing costs and lead times associated with traditional top-down assembly methods. This technology has been notably applied in TCR gene synthesis, showcasing its potential in advancing genetic research and development.

In addition to PathFinder DNA Assembly™, RootPath employs the Molecular Toothpick™ technology, which enhances the accuracy of gene synthesis by screening for errors in synthetic DNA strands. This technology addresses the common issue of errors in short synthetic DNA, which can occur at a rate of 1 in 100 bases. By examining each base pair of the assembled gene products, Molecular Toothpick™ selectively recovers error-free molecules without the need for extensive bacterial colony screening infrastructure. Together, these technologies position RootPath as a leader in enabling scalable and precise synthetic biology applications.

United States

RootPath is a synthetic biology company that elevates the throughput of gene function interrogation by orders of magnitude.  Our molecular programming-based DNA fragment assembly technology gives life science researchers, drug developers and synthetic biologists unprecedented access to accurate, long synthetic genes and their functional readouts. We enable biology at scale.

RootPath

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