The Yale Keck DNA Sequencing Lab employs advanced technologies to provide efficient and cost-effective DNA sequencing services. Central to their operations are the Applied Biosystems 3730xL DNA Analyzers, which utilize fluorescence-based detection methods to accurately determine the size of DNA fragments. The lab offers a variety of Sanger sequencing services, including individual tube sequencing, high-volume plate sequencing, and PCR purification. Additionally, they provide specialized services such as human cell line authentication and DNA and RNA quality control analysis, ensuring that researchers receive reliable and high-quality data for their projects.

In terms of fragment analysis, the lab requires samples to be submitted in specific 96-well optical plates and utilizes various dye sets compatible with their instrumentation. The standard turnaround time for results is one business day, with priority given to Yale users. The facility also supports the analysis of microsatellite data and offers software options like GeneMarker and GeneMapper for result interpretation. Overall, the Keck DNA Sequencing Lab is committed to maintaining high standards of quality and efficiency in their sequencing and analysis services, catering to both internal and external researchers.

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Yale Keck DNA Sequencing Lab

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